Researchers from the Netherlands found that a human antibody 47D11 to S-protein on the surface of the virus SARS-CoV and neutralizes SARS-CoV-2 in the cultures, preventing it to attach to cells and penetrate them. Article submitted for peer review in Nature, with its a Preprint is available on biorxiv.org.
SARS-CoV-2, which was the cause of the current pandemic, the structure is very similar to SARS-CoV that causes severe acute respiratory syndrome (“SARS”), whose outbreak occurred in 2002-2003. In the framework of the fight against the epidemic was developed some potential agents against SARS-CoV, however, to conduct their clinical trials failed: decreased and has become inadequate for such tests, the number of infected.
Now, Berend-Jan Bosch (Berend-Jan Bosch), Utrecht University and Frank Grosveld (Frank Grosveld) Medical center Rotterdam Erasmus University have tested a monoclonal antibody to S-protein on the surface of SARS-CoV, they got about 15 years ago and since then kept in a frozen state, as the epidemic caused by the virus at that time already over.
Antibodies, in particular 47D11 received from the hybrid transgenic mice and introduced into cell cultures green monkey VeroE6 and cells of the human kidney HEK-293T, pre-infected with viruses vesicular stomatitis with S-protein from SARS-CoV or SARS-CoV-2 on the surface. Next, enzyme-linked immunosorbent assay checked, joined, whether 47D11 to S-protein of viruses.