scientists have introduced into the ASP gene from Penicillium xiloglucanes streaks of gray, whereby the trees may be able to achieve the desired cutting size of 10 years earlier than usually reported in BMC Plant Biology. Transgenic aspen wood also decomposes almost two times slower than wild-type plants. Such trees are useful for restoration of forests in paper and wood industry.
Despite the abundance of artificial materials, natural still widely used. For example, the international stock volume of wood grows every year. In this regard, you must, first, quickly resume the forest, and secondly, to increase the productivity of plantations, which are going to cut: this allows you to use a smaller area. Across Eurasia grown aspen (aka poplar trembling, Populus tremula), so it is advisable to improve the breed. However, to do so by traditional breeding, choosing the most fast-growing trees and crossing them, for a very long time. To speed up the process of genetic engineering, but need to know what genes should act.
Although trees grow indefinitely, speed of increasing of their sizes is limited primarily by the structure of the cellulose membranes of the cells. Its rigidity is ensured, in particular, polysaccharides xyloglucan, and that cell could become more necessary to break a portion of the bonds in their molecules. Doing this the enzymes xiloglucanes. If Osin has been improved xiloglucanes their cage could stretch harder. Respectively, would have increased and the growth rate of the whole tree.
The staff of the Pushchino branch of Institute of Bioorganic chemistry, Institute of chemistry, Komi Scientific center, Ural branch of RAS and Siberian Federal University under the direction of Konstantin Shestopalov (Konstantin A. Shestibratov) created 25 lines of transgenic aspens in which the introduced recombinant gene xiloglucanes sp-Xeg of streaks of gray Penicillium (Penicillium canescens), and two control lines, wild type and gene beta-glucuronidase. Mushrooms powerful xyloglucans need to penetrate hyphae in plant tissue.
Aspen each line, for 50 copies, was obtained by vegetative propagation (cloning) from the respective prototype. Within two months they were kept in a greenhouse and then representatives of the six most rapidly growing lines of four months was transferred to the open ground at the site of Pushchino Institute. After the part of the plant left to spend the winter on the street. The researchers estimated the size of seedlings, the total area and weight of the leaves, the composition of the cell walls, the rate of decomposition of wood for six months (he had to dig up a few aspens), the expression level of the gene sp-Xeg and some other parameters.