Scientists from China infected artificial intestines (interaide) human and bat coronavirus SARS-CoV-2. For this asteroidy bats first raised in the culture. The virus actively reproducing in infected cells; it is found in the feces of patients with COVID-19. The authors of an article published in the journal Nature Medicine, concluded that SARS-CoV-2 can cause an intestinal infection in addition to respiratory. It remains unclear how the virus enters the intestine and can be transmitted the fecal-oral route.
In addition to fever, cough, difficulty breathing and other respiratory symptoms, some patients with COVID-19 there are signs of a gastrointestinal infection: nausea, vomiting, and diarrhea. Viral RNA was detected not only in the analysis of the respiratory tract, but also in the feces. To date, however, has not been proven that the virus infects the intestine and multiplies in it, although research carried out actively. On the same day that an article in Nature Medicine, the journal Science Immunology published a study of a group of scientists from the United States, where the artificial intestine (aneroid) of a person infected with coronavirus SARS-CoV-2, and showed that the virus multiplies in these cells.
Coronavirus SARS-CoV-2, which causes COVID-19, found in bats. These animals serve as a natural reservoir for many viruses in their feces and anal smears found several coronaviruses. SARS-CoV-2 similar to other coronaviruses of bats, the scientists believe that the new virus appeared in these animals. However, until now none of the coronavirus failed to isolate and cultivate the cells of bats.
A group of scientists from China, under the leadership of the young Juana Kwok (Kwok Yung Yuen) from a Hong Kong University have identified cells in the intestinal podkovonos bats Rhinolophus sinicus and people, including grown asteroidi — artificial thin intestines. These organelles resemble the cellular structure of the intestinal epithelium, for the bats they were first grown in culture.
Asteroidy infecting viruses from swabs of the nasopharynx and sputum of three patients with COVID-19. It proliferates in the cells was evaluated by the number of viral RNA and immunofluorescence staining of nucleocapsid protein. In addition, check to see if the intestinal cells of two human proteins (angiotensin converting enzyme 2, АПФ2, and serine protease TMPRSS2) that are needed in SARS-CoV-2 to enter the cell.
SARS-CoV-2 infected organelles, and viral burden increased over time (four orders of magnitude in 64 hours). Environment of the culture in which they thrived the virus has infected other asteroida, and SARS-CoV-2 multiplied again. In differentiated entroido found АПФ2 and TMPRSS2. So, the virus can infect cells of the intestine, multiply in them, and then re-infect another bowel.